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A sensible experiment spreads doses across at least three orders of magnitude, something like 1, 10, and 100 nanomolar on up to 1 and 10 micromolar, precisely because the sweet spot moves with the copper-to-peptide ratio, the buffer, and how much serum sits in the media
Colon Rectum 33, 891901
Its also remarkably stable unlike many peptides that degrade in acidic conditions, BPC-157 resists low-pH environments, an unusual structural property in its class
The steady-state plasma concentrations of fluoxetine and norfluoxetine increased by about 18% and 36%, respectively, and concentrations of paroxetine decreased by about 27%
Let's start with understanding how peptides actually relieve pain