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The organic acids were separated and identified using a Bio-Rad column (Aminex HPX-87H) with an Agilent diode array detector at 65 C, 5 mM H 2 SO 4 as mobile phase, 210 nm measurement wavelength, and a 0.6 mL/min flow rate that was increased to 0.8 mL/min at 60 min

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The soluble protein fraction was collected by centrifugation (15,000 g for 20 min at 4 C), and protein quantification was performed with a BCA assay

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Many of the experiments reviewed here rely on pharmacologic G6PD inhibition or G6PD siRNA knockdown