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Under physiological conditions, more than 98% of the total GSH pool exists in the reduced form, thereby shaping cellular redox potential and influencing proliferation, differentiation, and cell death 5 .Although the GSH/GSSG ratio has long been regarded as an indicator of cellular redox status, recent live-cell studies using genetically encoded redox sensors indicate that increases in GSSG are typically transient and compartmentalized rather than sustained intracellular accumulations 6,7

In contrast, treatment with KW6002 markedly increased these indices compared to the HY group (Fig

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Nine residues is long enough to be a comfortable substrate for endopeptidases as well as exopeptidases, so in serum-containing medium over a long incubation the concentration falls