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Cas9 protein, crRNA, and tracrRNA were assembled to ctRNP using protocols described previously 43

The C677T variant results from a single nucleotide substitution at this position, in which cytosine is replaced by thymine resulting a conversion of alanine to valine residue 24

The researchers subcutaneously injected the mice in the treatment group with 1.0 microgram/mouse of epitalon on 5 consecutive days each month