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Cells were washed twice with 1 PBS, trypsinized and resuspended in 3 ml of PBS and the fluorescence was immediately read on a Becton Dickinson FACScan with Cell Quest software (BD Biosciences, San Jose, USA)

The ratio of GSH to GSSG indicates your body's level of oxidative stress

doi: 10.1007/s11356-020-09088-2

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FOXO4-DRI at 25 mM concentration selectively induced p53 nuclear exclusion and apoptosis in the senescent Leydig cells, confirmed by Annexin V-FITC/PI apoptosis assays and immunofluorescent staining of Ser15-phospho-p53 foci