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After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

for example, vitamin C is known to regenerate vitamin E from its oxidized form (see the article on Vitamin E)

Amelioration of TMAO through probiotics and its potential role in atherosclerosis

Methyl 5-MeO-: N -aminoanthranilate, a minimalist fluorogenic probe for sensing cellular aldehydic load

Acta 1758 , 18641884 (2006)

The clinical backdrop is what follows