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Some protocols extend to 8-12 weeks with longer off periods

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Samples were then centrifuged at 1,252 rcf at 1C for 10 min, and the upper aqueous phase was carefully collected and dried at 4C using a refrigerated centrifugal concentrator (Labconco Refrigerated CentriVap Benchtop Vacuum Concentrator)

Genomic sections displaying sequence conservation over time within a single viral species or evolutionary conservation between members of the coronavirus family despite high mutation rates are indicative of essential regions of the viral genome that are functionally constrained, such as the fusion peptide within the spike S2 region [68, 69]