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This modified peptide contains 83 amino acids and exhibits reduced binding affinity to IGF binding proteins, making it particularly valuable for in vitro cellular research applications

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1 HNMR (300 MHz, DMSO- d 6 , ppm): H 11.58 (s, 1H, hydrazine-N H ), 9.74 (s, 1H, dimethoxybenzylidene-N=C H ), 8.39 (s, 1H, 4-hydroxy-3-methoxy benzohydrazide-O H ), 7.50 (s, 1H, 3,4-dimethoxybenzylidene-C 2 - H ), 7.39 (s, 1H, 4-hydroxy-3-methoxy benzohydrazide-C 2 - H ), 7.18 (brs, 1H, 4-hydroxy-3-methoxy benzohydrazide-C 6 - H ), 7.02 (m, 2H, 3,4-dimethoxybenzylidene-C 6 - H and 4-hydroxy-3-methoxy benzohydrazide-C 5 - H ), 6.89 (d, J = 6.0 Hz, 1H, 3,4-dimethoxybenzylidene-C 5 - H ), 3.85 (s, 3H, 4-hydroxy-3-methoxy benzohydrazide-OC H 3 ), 3.81 (s, 6H, 3,4-dimethoxybenzylidene-2(OC H 3 ))

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doi: 10.1523/JNEUROSCI.6577-10.2011