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Under normal conditions, Nrf2 is degraded through its interaction with Kelch-like ECH-associated protein 1 (Keap1), whereas under oxidative stress conditions, Keap1 is oxidized and Nrf2 is translocated to the nucleus, where it induces the expression of several genes 19

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The cultured SGN explants from WT or gpx1 / mice were exposed to peroxynitrite with or without ebselen pretreatment, and the expression changes of 4-HNE were examined to evaluate the oxidative stress level in SGNs (Figure 5A)

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