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ATP production was determined by enzymatically coupling ATP production to NADPH synthesis, with the reaction monitored using a Horiba Fluorolog-QM (Horiba Scientific) as previously described (21)

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Especially, patients with minimal hepatic encephalopathy showed a lower increase of cerebral oxyhemoglobin concentration in response to word fluency task than that of patients with liver cirrhosis without minimal hepatic encephalopathy 37