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The homogenate was vortexed for 30 min to ensure complete homogenization

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Furthermore, a study found that intraperitoneal injection of GLP-1 (Beinaglutide Injection) alleviates tissue injury effects in DSS-induced colitis mice by inhibiting intestinal inflammation, preserving the intestinal barrier, and modulating intestinal microbiota [219]

A key challenge in studying native GLP-1 is its extremely short in vivo half-life of approximately 12 minutes, owing to rapid inactivation by dipeptidyl peptidase-4 (DPP-4), which cleaves the N-terminal His-Ala dipeptide from GLP-1 to produce the inactive metabolite GLP-1(936)amide