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Consistent injection technique and regular dosing schedule support this tolerance-building process
High-density lipoprotein-based contrast agents for multimodal imaging of atherosclerosis
10.1136/jmedgenet-2015-103023 J
For wild-type or mutant hFSP1 expression in HEK293T or HT1080 hFSP1 KO cells, total RNA was extracted from HT1080 cells by using TRIzol Reagent (Ambion), then reverse transcription was performed to generate cDNA from total RNA by using HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme, China), then the genes of the full-length and truncation mutants of hFSP1 was amplified from the synthesized cDNA by standard PCR procedure, and subcloned into the pcDNA3.1 (Invitrogen)
62 (3), e01900-17
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