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The reporter of Cas12f with sgRNA for TTR was also constructed under the CMV promotor of pKLV2 vector, which has mCherry followed by T2A and TTR target sequence, further followed by the frameshifted two GFP sequences, IRES, and hygromycin resistance gene, resulting in pKLV2mCherry_TTR_GFPGFPv3

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Data Collection Data were extracted from the included studies using a standardized data extraction form by 2 reviewers

Researchers should follow appropriate laboratory handling and storage protocols

For a more detailed background, you can refer to upcoming resources on what peptide therapy is (article pending publication)

When intake explains the result and the patient is well, with no concerning clinical or laboratory findings,it can often be attributed as the likely cause without an extensive search for disease