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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

Fragments per kilobase of exon per million reads mapped were calculated for each gene using Cufflinks, and read counts for each gene were obtained using htseq-count

doi: 10.1073/pnas.1412271111

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