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For the analysis of epithelial cell trajectories in fetal and pediatric/adult stages, RNA velocities of single-cells were estimated based on unspliced and spliced mRNA information using Velocyto 37 , and RNA velocities were computed using the scVelo 0.3.2 package 149 implemented in Scanpy 1.9.8

Giunchi, V., Fusaroli, M., Hauben, M., Raschi, E., & Poluzzi, E

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