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The mixture was incubated in the dark with agitation for 10 min, and then the absorbance at 517 nm was measured using a multifunctional microplate reader

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Without adequate glutathione stores, Phase 1 toxic intermediates accumulate in the liver and bloodstream, causing severe systemic inflammation and triggering hypersensitivity reactions commonly seen in patients with complex chronic illnesses

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During ischemia, the absence of oxygen as the terminal electron acceptor halts oxidative phosphorylation and forces cells into anaerobic glycolysis

& Potter, J