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10.1126/science.aas9090 24 MartinsR.Lithgow GordonJ.WolfgangL

Mechanisms of Action

Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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