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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mM butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, Valencia, CA, USA) and sonicated for 30 sec in an ice bath (Ultrasonic bath, Branson, MO, USA)

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Interestingly, GSSG can also be reduced by NADPH

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Bacteriostatic Water Sterile Diluent for Peptide Reconstitution & Laboratory Preparation Bacteriostatic Water is a sterile, non-pyrogenic diluent commonly utilized in laboratory and research environments for the reconstitution and preparation of peptide-based research compounds