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Histopathological examination of kidney tissues Three left kidney samples were randomly selected for each group and fixed in 4% paraformaldehyde (PFA) for 48 h at room temperature

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The protein was added in 250 L of each of the pH clamped buffers in Eppendorf tubes and vortexed briefly

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Raiter A, Bechor Z, Kleiman M et al (2010) Angiogenic peptides improve blood flow and promote capillary growth in a diabetic and ischaemic mouse model

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Magnetic resonance spectroscopy (MRS) study shows increased E/I balance associated with decreased GABA level in the frontal lobe of autism [16]

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Regular monitoringincluding weight/BMI, HbA1c (for diabetes patients), blood pressure, and renal functionis essential throughout long-term therapy

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