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You should not use the water after this date, as its sterility and the preservatives effectiveness can no longer be guaranteed

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Biochimica Biophysica Acta 1407 (3), 205214

The percentage of spontaneous alternation behavior was determined as following: In vivo microdialysis Animals were anesthetized with inhalant isoflurane, and a microdialysis probe (MAB 9.14.2

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In a recent paper by Pascaud et al [11], a three-layered gradient of 1.10, 1.15 and 1.30 g/ml (2 ml of each) was produced by dilution of OptiPrep with 0.25 M sucrose, 6 mM EDTA, 60 mM Tris-HCl, pH 7.4 and the soil suspension (5 ml) layered on top