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The cells were grown at 27 C with shaking at 170 rpm for 72 h, at the end of which the cells were harvested by centrifugation and stored at 80 C

This task necessitates a thorough understanding of the factors influencing zinc metabolism in animals, including the effects of various feed components on zinc bioavailability and the distinct zinc requirements during critical physiological processes and states including growth, reproduction, and immune challenge [169]

Recent research has discovered several antivirulence agents

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