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14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20

doi: 10.1038/s41380-020-0645-4Google ScholarPubMed Pulvirenti, G., Valerio, C., Spadaro, F., D'Agata, V., Freni, V., Nardo, L., & Drago, F

Mullen KD, Gacad R: Pathogenetic mechanisms of acute hepatic encephalopathy

Petrelintide exhibited dose proportionality across the full range tested, supporting its suitability for once-weekly dosing

Within this framework, the etiologies of these disorders become clearer

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