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High-dose methionine (above 100 mg per injection) can elevate homocysteine levels in susceptible individuals, increasing cardiovascular risk if used long-term without folate co-supplementation

It is also contraindicated for epidural or spinal (intrathecal) injection procedures

Metabolite pathway analysis was conducted in the Pathway Analysis module of MetaboAnalyst 6.0

Injection Technique Subcutaneous (or intramuscular) injection technique, following established clinical practice and CDC guidance [13] [14]