2.8 Pull-down experiments Chloroplast extracts (100 g of Chl) were solubilized with 1% (w/v) dodecyl maltoside (DM) for 10 min at 4C, and incubated with 50 g of either purified His-TRX-f1, His-TRX-m1 and His-NTRC as bait proteins in binding buffer (BF, 25 mM Tris-HCl [pH 7.8], 150 mM NaCl, 5 mM MgCl 2 , 10% [v/v], glycerol, and cOmplete protease inhibitor [Roche]) overnight at 4C and 45 rpm
[PubMed] [Google Scholar] 79.Ilic S., Drmic D., Franjic S., Kolenc D., Coric M., Brcic L., Klicek R., Radic B., Sever M., Djuzel V., et al
Vigorous mixing or agitation can damage peptide structures
Its androgenic effects are like exogenous androgens thereby causing decreases in FSH and LH secretion which can negatively impact sperm production
Expression of glycosaminoglycans and small proteoglycans in wounds: modulation by the tripeptidecopper complex glycyl-lhistidyl-l-lysine-Cu2+
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