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As a rationale, the study proposed the evidence that stimulation of the Caco-2 cells with 10100 M BPC 157 resulted in an earlier, reproducible stimulation of the expression of mRNA for egr-1, with a peak after 15 min

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After centrifugation (20,000 g for 10 min), the supernatant was analyzed by western blotting with monoclonal anti-V5 (SV5-Pk1, Bio-Rad, Hercules, CA, USA, RRID:AB_322378) or monoclonal anti--actin antibody (2D4H5, Proteintech, Rosemont, IL, USA, RRID:AB_2687938)

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