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Western blot Cells were lysed in 1 RIPA buffer (Thermo Scientific, 89900) supplemented with cOmplete mini EDTA-free protease inhibitor tablets (Roche, 04693159001) and PhosSTOP phosphatase inhibitor tablets (Roche, 04906837001) and incubated on ice for 10 min

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The RNAi clones were obtained from RNAi feeding libraries created by Julie Ahringer and Marc Vidals laboratories 75,76

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Proponents on the panel said compounded peptides would be safer for consumers because compounding pharmacies are regulated by states and subject to FDA oversight