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After three or four freeze-thaw cycles, significant degradation has occurred

Therapeutic opportunities of GPBAR1 in cholestatic diseases

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[DOI] [PMC free article] [PubMed] [Google Scholar] Yoshida S, Tamaoki M, Shikano T, et al

Other means are available for diagnosing the presence of B12 malabsorption caused by PA, the autoimmune disease that destroys the cells producing intrinsic factor

the contents are 3x as much as in a normal shot which is just likely to be excessive and result in more being flushed down the lavatory as the rate of loss is higher if you have more in your blood so more isn't necessarily better - and they are also suggesting a really high frequency of use which is also likely to be unnecessary