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The lysate was filtered with a 0.45 m filter and loaded onto a Ni-NTA (Carl Roth, Germany) gravity column equilibrated with buffer A (20 mM sodium phosphate pH 7.5, 500 mM NaCl and 12.5 mM imidazole)

Across both trials, the reported safety profile was described as consistent with prior experience for the compound class, with gastrointestinal adverse events predominating

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Weight-Based Dosage Recommendations Weight plays a pivotal role in determining the appropriate body weight glutathione IV dosage