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After repeated washing the bound fraction was eluted with 250 mM imidazole (Fischer) in lysis buffer containing 10% glycerol (Acros)

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GGT is an heterodimeric glycoprotein located on the external plasma membrane of specific cells present in kidney tubules, biliary epithelium and brain capillaries where it hydrolyses GSH into glutamic acid and cysteinyl-glycine

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Eggersdorfer, M., & Wyss, A