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FIGURE 4 Validation of RNA-seq results and the expression analysis of Wnt/-catenin signaling pathway-related genes To verify the results of RNA-seq, 13 differentially expressed genes, including 6 downregulated and 7 upregulated genes, were randomly selected for qRT-PCR assay

-actin was used as the loading control

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Cats with one of these diffuse diseases can be sampled randomly using any one of these commonly employed techniques: ultrasound-guided fine-needle aspirates (FNA), ultrasound-guided needle biopsy, laparoscopic biopsies, or biopsies obtained surgically