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Diabetes 54 (8), 23142319 (2005)

After treatment, cells were lysed with lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1% Triton, 1 PIC), and treated with recombinant His8-USP7 (E-519-025, Bio-techne) at a final concentration of 20 nM for 6 hours at 37C

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Sterile Syringe: A 3ml or 5ml syringe is typically ideal for drawing and transferring the diluent