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When required, erythromycin (10 g/mL for S

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HCT-116 CRC cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1% (2 mM) L-glutamine and 1% (100 U/mL) Penicillin/streptomycin (complete medium) in an incubator at 37 C with 5% CO 2