The handling and storage guidance below reflects standard practice in published peptide research literature
Xu XY, Deng CQ, Wang J, Deng XJ, Xiao Q, Li Y, He Q, Fan WH, Quan FY, Zhu YP, Cheng P (2017) Plasma levels of soluble receptor for advanced glycation end products in Alzheimers disease
Its thiol side chain often participates in enzymatic reactions, and contributes to the stability of proteins by forming disulfide bonds
However, these trials were never published in detail in peer-reviewed journals
& Chandel, N
The insoluble matrix was then resuspended in reducing 2% (w/v) SDS lysis buffer, diluted from 3X SDS Blue Loading Buffer (New England Biolabs) + 5% 2-Mercaptoethanol (Sigma-Aldrich), and boiled for 15 min