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Concentrated, buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris, 300 mM NaCl, pH 7.0) supplemented with either 0.01% LMNG/0.001%CHS or 0.06% digitonin and immediately used for biochemical assay or cryo-EM grid preparation following quantification by BCA Assay (Pierce)

Huynh, K

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But to get to this point, it took a lot of basic science, which is focused on learning about the world