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The OCR was measured in real-time, quantifying the picomoles of oxygen consumed per minute (pmol/min), and graphed over time, before and after the addition of Rotenone to the culture medium (baseline OCR and specifically used for measuring mitochondrial respiration)

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T cells alone were cultured with and without plate-bound anti-CD3/CD28 as a positive control for functional, viable T cells

In aging mice this could mean a failure to adapt to aging-related bioenergetics deficits with less flexibility in fuel use in E4s which is further aggravated by female sex