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Melanotan II acts as a non-selective agonist with nanomolar-range affinity at MC1R, MC3R, MC4R, and MC5R, with relative potencies varying by assay system but generally showing highest functional activity at MC1R and MC4R in cAMP accumulation assays using transfected cell lines

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Thus, a deficiency in this metabolite can contribute to a variety of physiological disturbances, including a weakened intestinal barrier, microbial translocation, and increased production of pro-inflammatory cytokines

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T.PawlickR.et al (2025)

Significant between-study heterogeneity ( I 2 = 709 %, P = 0002) was reduced by subgrouping by study location, treatment duration and type of control (Table 2)