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Using a continuous assay, we determined K M s of recombinant Cr GPDH as 45 3 M for NADH and 820 127 M for DHAP, while k cat s were 147 10 and 248 36 s 1 , respectively ( Figure 2e-f , Table 1 )

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Supernatants were removed and 100 L of DMSO was added to each well to solubilize the formazan crystals

This is consistent with the mitochondrial mechanism cardiolipin stabilization and ETC optimization take time to translate into measurable cellular energy improvement

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