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Currently, endpoint measurements are related to severe cell damage such as tetrazolium reduction assay and lactate dehydrogenase (LDH) assay to detect viable cells and IL-1 release as inflammatory markers 21

PMID 40759852

By week 24, total weight loss from baseline commonly falls in the 12-18% range, though individual results vary significantly based on starting weight, diet, exercise, and metabolic factors

experimental controls, pre-specified endpoints , and bias mitigation strategies should be implemented

Although intracerebroventricular (i.c.v.) administration of GH caused no significant changes in food intake during the first 4 h of measurement, C57BL/6 mice exhibited increased food intake and reduced energy expenditure 24 h after the injection (Fig

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