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That's basic immunology

Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken

doi: 10.1038/s41434-020-00205-x 22 MaWHeHWangH

Int J Mol Sci, 2021

The protein has been purified and characterized from a variety of organisms (Rao and Reddy, 2008

Media was removed 4 h later and cells were lysed with 40 l TNT lysis buffer (20 mM Tris, pH 8, 200 mM NaCl, 1% triton-100)