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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

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In brief, CTR-HEK 293 cells transiently transfected with GCGR or GPR119 were washed with phosphate buffered saline (PBS), resuspended in PBS containing 0.1% bovine serum albumin (BSA) plus 25M rolipram, and seeded at 2000 cells/well in 384-well white Optiplates

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The chemical structure of the DPPH radical (DPPH) is illustrated in Fig