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The samples were subsequently treated overnight at 4C with primary antibodies: RUNX2, OCN (1:1,000, Proteintech, China), GAPDH (1:3,000, Proteintech, China), Wnt3a, LRP5, and -catenin (1:1,000, Proteintech, China)

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NAD+ (nicotinamide adenine dinucleotide) is a vital coenzyme found in every cell of the body, essential for energy production, DNA repair, and healthy aging

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By increasing insulin production in response to high blood sugar levels and decreasing glucagon, which helps release glucose into the bloodstream, semaglutide effectively lowers and stabilizes blood glucose levels

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