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To implement effective site rotation: Divide each injection area (abdomen, thigh, or arm) into sections Keep a simple record or mental note of which section was used for each weekly injection Move at least 23 cm away from the previous injection point Avoid returning to the same specific spot for several weeks Patients should inspect injection sites regularly for any signs of persistent redness, unusual lumps, or other changes

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The remaining supernatant was heated at 70 C for 10 min

Faded dark spots, fewer breakouts, no more dry and flaky skin, no more dull skin