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PMID 37154744

For STED microscopy, Goat-Anti-Rabbit-IgG-Atto 647 N (Sigma-Aldrich) and Goat anti-Mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 594 (Thermo Fisher Scientific) were used at the 1:100 dilution

We therefore performed isothermal titration calorimetry (ITC) to determine the affinity of OTS964 to CDK11B p58 -cyclin L2-SAP30BP and the isolated CDK11B p58 monomer

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