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2.8 ELISA Pro-inflammatory cytokine profiling was performed by homogenizing colonic tissues in chilled phosphate-buffered saline (PBS), with the resulting supernatants harvested via centrifugation

This approach may protect glutathione from gastric acid and intestinal enzymes, potentially allowing greater intact absorption into the bloodstream

This is documentation, not a protocol to follow

Human growth hormone is a single-chain polypeptide that is secreted by the pituitary gland

Barbieri M, Ferrucci L, Ragno E, Corsi A, Bandinelli S, Bonafe M, Olivieri F, Giovagnetti S, Franceschi C, Guralnik JM, Paolisso G (2003) Chronic inflammation and the effect of IGF-I on muscle strength and power in older persons

The contributing factor may change from week to week, which is why tracking is so valuable