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After centrifugation at 15,700 g for 5 min the supernatant was discarded and the cell pellet was repeatedly washed in 5 mL of TE buffer and resuspended in 3 mL of lithium/caesium acetate solution (Qbiogene)

De Bleser PJ, Xu G, Rombouts K, Rogiers V, Geerts A: Glutathione levels discriminate between oxidative stress and transforming growth factor-beta signaling in activated rat hepatic stellate cells