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All experiments were performed according to the guidelines for using laboratory animals by ARRIVE guidelines (2019) and the US National Institutes of Health, as approved by the Research and Ethics Committee of Riphah Institute of Pharmaceutical Sciences, Riphah International University (Reference no: REC/RIPS/2022/06)

Thus, it is essential to achieve high payload delivery of H 2 at specific locations to improve the efficacy of H 2 intervention

Lyophilized powder remains stable for 12 to 24 months at standard refrigerator temperature

it's about understanding the concentration you need for your specific research protocol

Initially (untreated cells for initial time point) or after 4 days (with varying treatment conditions), 40 L of Cell Titer Glo reagent (Promega) was added to each well, mixed briefly, and the luminescence read on a luminometer (Molecular Devices)

Ethics statement Human studies gained approval (ID 2023-KY-030-01) from the Medical Ethics Committee affiliated to Beijing Obstetrics and Gynecology Hospital, Beijing Maternal and Child Health Care Hospital