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RNA isolation and real-time PCR Request a detailed protocol Total RNA was isolated from FACS-purified cells lysed with Trizol (Life Technologies) followed by extraction using a direct-Zol RNA mini prep kit (Zymo research)

Unlike hormone replacement therapies, it doesnt directly increase testosterone production

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Maleki, Z., Basirat, Z., Faramarzi, M., & Nasiri Amiri, F