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2) Dose Response: 60 l of 10 mM stock -OR- 1 mg (pre-weighed)

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Effects may be less dramatic than the first course if the initial burden was substantially reduced Maintenance: Some community protocols transition to once-yearly courses for ongoing senescent cell management There is no research to validate this approach

The injectate should proceed without any resistive pressure and often an expansion of fluid can be seen anterolaterally consistent with inflation of the bursa