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The fluorescence intensity of the blank control and the experimental group was measured under the appropriate exposure time and gain conditions, and the same detection system and measurement parameters were used in the measurement process to ensure the reliability and accuracy of the fluorescence signal
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Hou B, Zhou L, Wang H, Saeed M, Wang D, Xu Z, et al
To acclimate individuals to new environments, such as the behavioral test arenas for chemosensory research, animals are kept in these tanks for extended periods of time (hours or even days) prior to experiments, often without water change, filtration pumps, or oxygenation as these would create physical disturbance and currents
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