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After collection of the ascitic fluid, it was centrifuged at 1200 rpm for 2 min at room temperature, to remove the cells avoiding cell lysis

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Cytochemical SA--galactosidase assay Cells were seeded in six-well plates, fixed with 0.5% glutaraldehyde (Sigma, G5882) in PBS for 15 min, washed with 1 mM MgCl 2 /PBS (pH 6.0) and then incubated with X-Gal staining solution (1 mg ml 1 of X-Gal (Thermo Scientific, 2737087), 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 ) for 68 h at 37 C

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While the body produces L-Carnitine, it can also be obtained from dietary sources, predominantly red meat and dairy products

Iron, B vitamins, calcium, zinc, and vitamin D are commonly insufficient in low-calorie eating patterns